Fluoride-induced Oxidative Stress in Adrenal Gland of Rat

Fluorosis is a dreaded condition caused due to high exposure of sodium fluoride in drinking water. The aim of this study was to use sensitive biomarkers to evaluate damage to adrenal gland after fluoride exposure. Recently, weaned Wistar rats were exposed to 100, 200 and 300 ppm fluoride concentrations in drinking water for a period of 20 and 40 days. At the end of exposure period, oxidative stress parameters and activity of antioxidant enzymes were measured in adrenal tissue. The fluoride content in the adrenal tissue increased significantly (p<0.001) in all rats after 20 and 40 days of fluoride intoxication in comparison to control. The level of malondialdehyde in adrenal gland of test rats were also increased significantly (p<0.001) while antioxidant enzymes such as superoxide dismutase and catalase were reduced after sodium fluoride treatment. Glutathione concentration was significantly (p<0.001) decreased.